© 2013 | Antimicrobial Discovery Solutions Ltd
We have expertise spanning microbiology, biochemistry and structural biology and
specialising in the development of novel reagents and assays to support biological science and translational research:
We offer native and labelled (radioactive, fluorescent and NMR active) peptidoglycan reagents to from UDP-MurNAc sugar peptides to Lipid II.
The compound library begins with the cytoplasmic intermediate, enol pyruvoyl UDP GlcNAc and extends to the lipid linked, undecaprenyl pyrophosphoryl MurNac (N-acetyl glucosamine) pentapeptide. The biosynthesis of these precursors in-vitro produces products of high purity, which are tested enzymatically and identity confirmed via mass spectrometry.
Single channel to 1536 plate assays in various formats with Anisotropy, TR-FRET, Fluorescent, luminescent and Absorbance output.
Assay expertise in
- Mur ligase ATPase &
Penicillin binding proteins (peptidoglycan synthase)
(see Lloyd et al (2013) Adenosine Tetraphosphoadenosine Drives a Continuous ATP-Release Assay for Aminoacyl-tRNA Synthetases and Other Adenylate-Forming Enzymes. ACS chemical biology 8: 2157-2163)
University Of Warwick, Gibbet Hill Road, Coventry, United Kingdom
Antimicrobial Discovery Solutions Ltd